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Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces
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Bioengineering
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JoVE Journal Bioengineering
Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces

Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces

DOI:
10.3791/57262-v

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07:01 min

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May 22, 2018

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, , ,
1Division of Biological Science, Graduate School of Science,Nagoya University, 2Institute of Transformative Bio-Molecules (ITbM),Nagoya University

Chapters

  • 00:05Title
  • 00:28Fabricating the PDMS Microdevices
  • 02:11In Vitro Culturing of T. fournieri Pollen Tubes in the Microdevice
  • 03:31In Vitro Culturing of A. thaliana Root Hairs in the Microdevice
  • 04:40In Vitro Culturing of P. patens (moss) Protonemata in the Microfluidic Device
  • 05:31Results: Microfluidic Device Design and Time-lapse Imaging of Pollen Tube Growth
  • 06:37Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method to investigate the capability of tip-growing plant cells, including pollen tubes, root hairs, and moss protonemata, to elongate through extremely narrow gaps (~1 µm) in a microfluidic device.

Tags

Keywords: Microfluidic DevicesTip-growing Plant CellsPollen TubesMoss ProtonemataRoot HairsCell BiologyPDMSAir PlasmaGrowth MediumPollen GrainsPollinated StyleMicroscopy

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