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Bioengineering
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微流控装置在极小空间中研究尖端生长植物细胞的伸长能力
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Bioengineering
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JoVE Journal
Bioengineering
Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces
Please note that all translations are automatically generated.
Click here for the English version.
微流控装置在极小空间中研究尖端生长植物细胞的伸长能力
DOI:
10.3791/57262-v
•
07:01 min
•
May 22, 2018
•
Naoki Yanagisawa
,
Nagisa Sugimoto
,
Tetsuya Higashiyama
2
,
Yoshikatsu Sato
1
Division of Biological Science, Graduate School of Science
,
Nagoya University
,
2
Institute of Transformative Bio-Molecules (ITbM)
,
Nagoya University
Chapters
00:05
Title
00:28
Fabricating the PDMS Microdevices
02:11
In Vitro
Culturing of
T. fournieri
Pollen Tubes in the Microdevice
03:31
In Vitro
Culturing of
A. thaliana
Root Hairs in the Microdevice
04:40
In Vitro
Culturing of
P. patens
(moss) Protonemata in the Microfluidic Device
05:31
Results: Microfluidic Device Design and Time-lapse Imaging of Pollen Tube Growth
06:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一种方法来调查尖端生长的植物细胞, 包括花粉管, 根毛和苔藓 protonemata, 以拉长通过极窄的差距 (约1µm) 在微流控装置。
Tags
Keywords: Microfluidic Devices
Tip-growing Plant Cells
Pollen Tubes
Moss Protonemata
Root Hairs
Cell Biology
PDMS
Air Plasma
Growth Medium
Pollen Grains
Pollinated Style
Microscopy
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