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Utviklingen av Microfluidic enheter å studere forlengelse evnen til tips voksende anlegget celler i svært små områder
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces
DOI:

07:01 min

May 22, 2018

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Chapters

  • 00:05Title
  • 00:28Fabricating the PDMS Microdevices
  • 02:11In Vitro Culturing of T. fournieri Pollen Tubes in the Microdevice
  • 03:31In Vitro Culturing of A. thaliana Root Hairs in the Microdevice
  • 04:40In Vitro Culturing of P. patens (moss) Protonemata in the Microfluidic Device
  • 05:31Results: Microfluidic Device Design and Time-lapse Imaging of Pollen Tube Growth
  • 06:37Conclusion

Summary

Automatic Translation

Vi beskriver en metode for å undersøke evnen til tips voksende anlegget celler, inkludert pollen rør, rot hår, og moss protonemata, til å forlenge gjennom svært smale åpninger (~ 1 µm) i en microfluidic enhet.

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