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Efeitos duplos de fatores derivado de células de Melanoma na diferenciação de adipócitos de medula óssea
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1Department of Rheumatology, Ren Ji Hospital, School of Medicine,Shanghai Jiao Tong University, 2Department of Nephrology and Rheumatology,Yongchuan Hospital of Chongqing Medical University, 3Department of Medical Oncology, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College,Fudan University
Chapters
- 00:04Title
- 00:48Melanoma Cell-derived Factor Harvest and Bone Marrow-derived Adipocyte Induction
- 03:09Red Oil O Staining and Quantitative Polymerase Chain Reaction (qPCR) Analysis
- 04:20Bone Marrow Adipocyte-melanoma Cell Co-culture
- 05:54Results: Dynamic Bone Marrow-derived Adipocyte Regulation of Tumor Cells
- 06:41Conclusion
Aqui, apresentamos um sistema confiável e simples bidimensional coculture (2D) para estudar a interação entre as células do tumor e da medula óssea de adipócitos, que revela um duplo efeito de fatores derivado de células de melanoma em adipócitos a medula óssea diferenciação e também coloca um método clássico para o estudo mecanicista da metástase óssea.
