Journal
/
/
植物の効率的な方法で複数のキメラ蛍光融合タンパク質の共発現
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
DOI:

09:45 min

July 01, 2018

, ,

Chapters

  • 00:04Title
  • 00:38DNA Fragment Assembly and Construction of Protein Expression Cassettes
  • 02:03Construction of the Vector for Co-expression of Multiple Chimeric Fluorescent Fusion Chimeric Fluorescent Fusion Proteins in Plants
  • 03:30Biolistic-bombardment Mediated Transient Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in Plants
  • 07:20Results: Representative Images of Co-expression of Chimeric Fluorescent Fusion of VSR and SCAMP in Plant Cells
  • 08:40Conclusion

Summary

Automatic Translation

従来の方法の難しさを克服するために植物の複数のキメラ蛍光融合タンパク質の共発現手法を開発しました。タンパク質の共発現を達成するためにカセットを表現する複数の機能的に独立して蛋白質を含んでいる単一発現プラスミドを使用する利点がかかります。

Related Videos

Read Article