A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:38DNA Fragment Assembly and Construction of Protein Expression Cassettes
- 02:03Construction of the Vector for Co-expression of Multiple Chimeric Fluorescent Fusion Chimeric Fluorescent Fusion Proteins in Plants
- 03:30Biolistic-bombardment Mediated Transient Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in Plants
- 07:20Results: Representative Images of Co-expression of Chimeric Fluorescent Fusion of VSR and SCAMP in Plant Cells
- 08:40Conclusion
Vi har utviklet en ny metode for co uttrykke flere chimeric fluorescerende fusion proteiner i planter å overvinne vanskelighetene med konvensjonelle metoder. Det tar fordelen av benytter en enkelt utrykk plasmider som inneholder flere funksjonelt uavhengig protein uttrykke kassetter for å oppnå protein co uttrykk.
