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Høy følsomhet påvisning av Micrometastases generert av GFP Lentivirus-transduced Organoids kultivert fra en pasient-avledede kolon svulst
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1Department of Coloproctological Surgery,Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis,Juntendo University Faculty of Medicine, 3Atopy Research Center,Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery,Tokyo Dental College, 5Department of Gastroenterology,Juntendo University Faculty of Medicine
Chapters
- 00:04Title
- 00:57Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
- 02:42CRC Organic Cell-labeling with GFP Lentiviral Particles
- 05:28GFP-labeled CRC Organoid Metastasis Generation
- 07:55Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
- 08:38Conclusion
For at svært følsom påvisning av formidle menneskelige kolorektal kreft (CRC) celler kolonisere vev, viser vi her en protokoll for effektiv signaltransduksjon grønne fluorescerende protein (GFP) lentiviral partikler i PDX-avledet CRC organoid celler før deres injeksjon i mottakerens mus, med stereo-fluorescens mikroskopiske observasjon.
