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Patient-Derived Tumor Explants As a “Live” Preclinical Platform for Predicting Drug Resistance in Patients
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JoVE Journal Cancer Research
Patient-Derived Tumor Explants As a “Live” Preclinical Platform for Predicting Drug Resistance in Patients

Patient-Derived Tumor Explants As a “Live” Preclinical Platform for Predicting Drug Resistance in Patients

DOI:
10.3791/62130-v

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07:42 min

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February 07, 2021

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, , , , , , , , , , ,
1Leicester Cancer Research Center,University of Leicester, 2MRC Toxicology Unit

Chapters

  • 00:04Introduction
  • 00:45Explant Preparation and Drug Treatment
  • 02:12Histological Processing
  • 03:09Training Analysis
  • 06:04Results: Representative Patient-Derived Explant Responses to Tumor Drugs of Interest
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes methods for the generation, drug treatment, and analysis of patient-derived explants for assessing tumor drug responses in a live, patient-relevant, preclinical model system.

Tags

Keywords: Patient-derived Tumor ExplantsPreclinical PlatformDrug Resistance Prediction3D Tumor ContextDrug ResponseDrug MetabolismPharmacodynamic BiomarkersOrganotypic CultureDrug TreatmentFormalin FixationHistological Processing

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