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多路人工细胞微环境阵列的制备
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
DOI:

07:19 min

September 07, 2018

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Chapters

  • 00:04Title
  • 00:38Nanofiber Array Preparation
  • 03:06Fabrication of Microfluidic Structures and Assembly of MACME Arrays
  • 03:57Loading hESCs into MACME Array and Image Acquisition
  • 05:23Results: Analysis of Multiplexed Artificial Cellular Microenvironments for Human Pluripotent Stem Cells
  • 07:01Conclusion

Summary

Automatic Translation

本文介绍了用于制备多路人工细胞微环境 (MACME) 阵列的详细方法, 用于对模拟体内细胞微环境的物理和化学线索进行高通量操作, 并用单细胞分析方法确定人类多潜能干细胞 (hPSCs) 的最佳细胞环境。

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