/
/
Fabricação de uma matriz de microambiente celular Artificial multiplexado
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Institute for Integrated Cell-Material Sciences (WPI-iCeMS),Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology,Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering,Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources,Universiti Malaysia Sabah, 5Institute for Chemical Research,Kyoto University, 6Ecole Normale Supérieure
Chapters
- 00:04Title
- 00:38Nanofiber Array Preparation
- 03:06Fabrication of Microfluidic Structures and Assembly of MACME Arrays
- 03:57Loading hESCs into MACME Array and Image Acquisition
- 05:23Results: Analysis of Multiplexed Artificial Cellular Microenvironments for Human Pluripotent Stem Cells
- 07:01Conclusion
Este artigo descreve a metodologia detalhada para preparar uma matriz multiplexado Artificial celular microambiente (MACME) para manipulação de alta produtividade de física e química sugestões imitando na vivo o microambiente celular e para Identifica o ambiente celular ideal para as células-tronco pluripotentes humanas (hPSCs) com perfis de célula única.
