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胶质瘤移植中体内成像的荧光分子层析成像研究
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JoVE Journal Cancer Research
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
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胶质瘤移植中体内成像的荧光分子层析成像研究

DOI:
10.3791/57448-v

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07:52 min

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April 26, 2018

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, , , ,
1Ludwig Institute for Cancer Research, 2Moores Cancer Center, School of Medicine,University of California, San Diego, 3Department of Pathology, School of Medicine,University of California, San Diego, 4Department of Medicine, School of Medicine,University of California, San Diego

Chapters

  • 00:05Title
  • 00:55Construct-positive Glioblastoma Cell Sorting
  • 02:46Near-infrared Fluorescent Protein (iRFP)-tagged Glioblastoma Cell Intracranial Injection
  • 04:49Fluorescence Molecular Tomography (FMT) Imaging and Analysis
  • 06:22Results: Representative In Vivo FMT Imaging of Glioblastoma Xenografts
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

原位颅内注射肿瘤细胞已用于癌症研究, 研究脑肿瘤生物学, 进展, 进化和治疗反应。本文提出了肿瘤移植的荧光分子层析成像技术, 为临床前的胶质瘤模型提供了实时活体成像和肿瘤质量定量。

Tags

Keywords: Fluorescence Molecular TomographyFMTGlioblastoma XenograftsIn Vivo ImagingBrain Tumor BiologyCancer ResearchDrug DiscoveryTumor AggressivenessTumor HeterogeneityTreatment ResponsivenessNon-invasiveQuantitativeXenograft ModelsGlioblastoma CellsLentivirusFluorescent ProteinFlow CytometryCell SortingSingle Cell SuspensionPBSIn Vivo Experiments

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