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Orthotopic Homogref (Syngeneic) fare birincil KPC pankreas duktal adenokarsinom, akış sitometresi tarafından Immunophenotyping
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
DOI:

08:30 min

October 09, 2018

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Chapters

  • 00:04Title
  • 00:33Preparation for Tumor Transplantation
  • 01:40Surgical Operation for Orthotopic Implantation
  • 02:58Tumor Tissue Dissociation and Single Cell Preparation
  • 05:01Flow Data Acquisition
  • 06:46Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
  • 08:04Conclusion

Summary

Automatic Translation

Fare orthotopic PDAC homografts immunophenotyping deneysel ameliyat tümör IMMUNO-microenvironment profil oluşturma amaçlamaktadır. Tümör ameliyat ile implante orthotopically vardır. Tümör boyutu 200-600 mm3 hasat ve tek hücreli süspansiyonlar, çok bağışıklık marker FACS analizi farklı fluorescently etiketli antikorlar tarafından takip hazırlamak için ayrışmış.

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