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Avaliação pré-clínica da Bioatividade da OT48 de cumarina Anticancer esferoides, ensaios de formação de colônia e Xenografts Zebrafish
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Preclinical Assessment of the Bioactivity of the Anticancer Coumarin OT48 by Spheroids, Colony Formation Assays, and Zebrafish Xenografts
DOI:

09:20 min

June 26, 2018

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Chapters

  • 00:04Title
  • 00:33Colony Formation Assays
  • 03:47Zebrafish Egg Production and Incubation for Xenograft Assay
  • 05:05Compound-treated Cell Preparation and Microinjection of Cancer Cells for Xenograft Formation
  • 07:21Results: Inhibitory Potential of Anticancer Compounds on Spheroid Formation and In Vivo Tumor Formation in Zebrafish
  • 08:29Conclusion

Summary

Automatic Translation

Aqui, apresentamos o rastreio pré-clínico de cumarinas anticâncer usando zebrafish e cultura 3D.

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