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Retroductal Nanoparticle Injection to the Murine Submandibular Gland
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Bioengineering
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JoVE Journal Bioengineering
Retroductal Nanoparticle Injection to the Murine Submandibular Gland

Retroductal Nanoparticle Injection to the Murine Submandibular Gland

DOI:
10.3791/57521-v

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07:45 min

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May 03, 2018

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, , , , , ,
1Department of Biomedical Engineering,University of Rochester, 2Department of Otolaryngology Head and Neck Surgery,University of Rochester Medical Center, 3Center for Oral Biology,University of Rochester Medical Center

Chapters

  • 00:05Title
  • 00:44Experimental Preparation
  • 01:40Accessing and Visualizing Ductal Entry Point
  • 02:39Ductal Cannulation and Line Placement
  • 05:04Injection
  • 05:52Results: Confirmation of Retroductal Nanoparticle Persistence 24 Hours Post Injection
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

Local drug delivery to the submandibular glands is of interest in understanding salivary gland biology and for the development of novel therapeutics. We present an updated and detailed retroductal injection protocol, designed to improve delivery accuracy and experimental reproducibility. The application presented herein is the delivery of polymeric nanoparticles.

Tags

Nanoparticle InjectionSubmandibular GlandRetroductal AdministrationMurine ModelSalivary Gland DysfunctionRadiation TherapyHamilton SyringeCannulationAtropineDissecting MicroscopeTongue ManipulationDuctal Access

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