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CRISPR 指南对小鼠前列腺病毒传递的基因改变
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
DOI:

06:21 min

April 27, 2018

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Chapters

  • 00:04Title
  • 00:47Surgical Preparations
  • 01:40Virus Delivery to the Murine Prostate
  • 04:35Results: Loss of Pten Expression in the Prostate Using CRISPR/Cas9 Gene Editing
  • 05:45Conclusion

Summary

Automatic Translation

该协议描述了一种新建立的方法, 病毒传递到小鼠前列腺。使用 CRISPR/Cas9 技术, 基因过度表达, 或 recombinase 分娩, 该技术允许基因表达的原位改变, 并实施了新的小鼠模型前列腺癌。

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