Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Preparation of Exosomes for siRNA Delivery to…
JoVE Journal
Cancer Research

This content is Open Access.

JoVE Journal Cancer Research
Preparation of Exosomes for siRNA Delivery to Cancer Cells
 

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Article DOI: 10.3791/58814-v • 09:59 min • December 5th, 2018
December 5th, 2018 •
*1, *1, 1
1Institute of Pharmaceutical Science, King's College London
* These authors contributed equally

Chapters

Summary
Automatic Translation

An exosome is a new generation of drug delivery carriers. We established an exosome isolation protocol with high yield and purity for siRNA delivery. We also encapsulated fluorescently labelled non-specific siRNA into exosomes and investigated the cellular uptake of siRNA-loaded exosomes in cancer cells.

Tags

Keywords: Exosomes SiRNA Delivery Cancer Cells Bioreactor Ultracentrifugation Sucrose Cushion HEK-293 Cells Exosome-depleted Medium Pre-clearing Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter