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用于向癌细胞输送 sirna 的外质体的制备
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Preparation of Exosomes for siRNA Delivery to Cancer Cells
DOI:

09:59 min

December 05, 2018

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Chapters

  • 00:04Title
  • 00:51Bioreactor Flask Cell Culture
  • 01:52Conditioned Medium (CM) Harvesting from the Bioreactor Flask
  • 02:41Exosome Isolation onto a Sucrose Cushion
  • 05:02siRNA Encapsulation into Exosomes by Electroporation
  • 06:11Removal of Free siRNA Using Size Exclusion Chromatography (SEC)
  • 07:17Results: Effective Intracellular Delivery of siRNA Using siRNA-encapsulated Exosomes
  • 09:11Conclusion

Summary

Automatic Translation

外显子是新一代的药物输送载体。我们建立了一个具有高产量和纯度的非连续分离协议, 用于 sirna 的传递。我们还将荧光标记的非特异性 sirna 封装到外显子体中, 并研究了癌细胞中含有 sirna 的外显子体的细胞吸收情况。

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