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利用 Microrespiration 分析和显微解剖研究 Teliospore 萌发

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Biology

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JoVE Journal Biology

Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection

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利用 Microrespiration 分析和显微解剖研究 Teliospore 萌发

DOI:

10.3791/57628-v

•

08:50 min

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May 13, 2018

•

Lauren A. Ostrowski*¹, Amanda M. Seto*¹, Barry Saville²

¹Environmental and Life Sciences Graduate Department,Trent University, ²Forensic Science Department,Trent University

Chapters

00:04TItle
01:01Induction of Germination for Respiration Monitoring and Obtaining Oxygen Consumption Rate (OCR) Measurements
02:34Induction of Teliospore Germination to Isolate Teliospores at Distinct Stages of Germination and Preparation of Micromanipulator
04:34Isolation of Stage-specific Germinating Teliospores
06:01Results: Microrespiration Analysis and Microdissection for Assessing Teliospore Germination
07:32Conclusion

Summary

Automatic Translation

黑穗病真菌引起许多破坏性的农业病害。它们被分散为休眠冬孢子, 在响应环境提示时发芽。我们概述了两种方法来研究发芽过程中的分子变化: 测量呼吸增加, 以检测代谢活化和评估变化的分子事件通过隔离冬孢子在不同的形态学阶段。

Tags

Teliospore Germination Microrespiration Analysis Microdissection Respiration Level Gene Transcript Levels Pathogen Dissemination Corncob Infection Teliospore Viability Teliospore Suspension Oxygen Consumption Rate (OCR)Potato Dextrose Broth Streptomycin Sulfate Kanamycin Sulfate Erlenmeyer Flask

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