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Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

DOI:
10.3791/57640-v

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07:04 min

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September 16, 2018

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, , , , , ,
1Shanghai Public Health Clinical Center, Shanghai Medical College,Fudan University, 2Department of Medical Biotechnology,Yeungnam University, 3Department of Chemistry,Pukyong National University, 4Marine-integrated Bionics Research Center,Pukyong National University, 5Department of Biochemistry,Chungbuk National University, 6Department of Biomedical Sciences, University of Ulsan College of Medicine,Asan Medical Center

Chapters

  • 00:00Title
  • 00:16Amphiphilic Oligodeoxyribonucleotide to form DNA Nanoparticles
  • 01:44Administration of INP via Multiple Injection Routes
  • 02:56Isolation of Lymph Nodes and Spleen
  • 06:06Results and Conclusion

Summary

Automatic Translation

Automatic Translation

Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial.

Tags

Keywords: Immunostimulatory AgentDendritic Cell ActivationMediastinal Lymph NodeDNA AmphiphileNano-sized MicellesToll-like Receptor 9Immune-stimulating Nanoparticle (INP)Injection RouteImmunological AnalysisSpleenLymph Nodes

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