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Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue
DOI:

06:27 min

July 30, 2018

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Chapters

  • 00:04Title
  • 00:53Glass-supported Planar Lipid Bilayer Formation and Planar Bilayer: T Cell Interaction Imaging
  • 04:39Results: Representative T Cell: Lipid Bilayer Interaction Analyses
  • 05:33Conclusion

Summary

Automatic Translation

The protocol describes a technique to study the ability of primary polyclonal human T cells to form synaptic interfaces using planar lipid bilayers. We use this technique to show the differential synapse formation capability of human primary T cells derived from lymph nodes and peripheral blood.

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