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JoVE Journal Medicine
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
 

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Article DOI: 10.3791/57647-v • 08:55 min • May 18th, 2018
May 18th, 2018 •
1, 1, 1, 1
1Department of Clinical Sciences, North Carolina State University

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Summary
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This protocol will enable readers to successfully establish a porcine model of segmental intestinal ischemia and subsequently isolate and culture intestinal stem cells for the study of epithelial repair following injury.

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Keywords: Intestinal Stem Cells Ischemia Porcine Model Small Intestine Crypt Isolation Epithelial Stem Cells Tissue Repair Dissociation
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