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Medicine
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猪节段性小肠缺血模型中肠道干细胞的分离与培养
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
Please note that all translations are automatically generated.
Click here for the English version.
猪节段性小肠缺血模型中肠道干细胞的分离与培养
DOI:
10.3791/57647-v
•
08:55 min
•
May 18, 2018
•
Amy Stieler Stewart
,
John M Freund
,
Anthony T Blikslager
,
Liara M Gonzalez
1
Department of Clinical Sciences
,
North Carolina State University
Chapters
00:04
Title
00:44
Ischemia and Tissue Collection
02:54
Crypt Stem Cell Isolation
06:12
Results: Representative Crypt Isolation and Enteroid Generation
07:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议将使读者能够成功地建立猪节段性肠缺血模型, 随后分离和培养肠道干细胞, 用于研究损伤后的上皮修复。
Tags
Keywords: Intestinal Stem Cells
Ischemia
Porcine Model
Small Intestine
Crypt Isolation
Epithelial Stem Cells
Dissociation
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