A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Center for Mitochondrial and Epigenomic Medicine,Children’s Hospital of Philadelphia, 2Department of Cellular and Molecular Biology,University of Pennsylvania, 3Department of Biological Sciences,Mississippi State University, 4Center for Mechanisms of Evolution, Biodesign Institute,Arizona State University
Chapters
- 00:04Title
- 00:38RNA Circularization and Rolling Circle Reverse Transcription
- 02:51Second Strand cDNA Synthesis and Repair
- 03:45Adapter Ligation and Size Selection
- 06:21PCR Amplification and Final Bead Purification
- 07:53Results: Quality Control, Bio-Informatic Analysis, and Expected Results from the C-seq Assay
- 08:54Conclusion
