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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
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JoVE Journal Neuroscience
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

DOI:
10.3791/57790-v

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07:14 min

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August 16, 2018

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, ,
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Chapters

  • 00:04Title
  • 00:47Tissue Harvest and Section Acquisition
  • 03:06Perforated Patch-clamp and Electrophysiological Recordings
  • 05:39Results: Representative Pituitary Cell Current Clamp Recordings
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.

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