A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:50Mapping Fastq Files
- 02:14Bioinformatics Processing Using DEEPN Software
- 06:08Verification of Candidates Identified by DEEPN
- 08:01Conclusion
Deep sequencing of yeast populations selected for positive yeast 2-hybrid interactions potentially yields a wealth of information about interacting partner proteins. Here, we describe the operation of specific bioinformatics tools and customized updated software to analyze sequence data from such screens.
Tags
Keywords:
Informatic AnalysisSequence DataBatch Yeast 2-hybrid ScreensProtein InteractomesBioinformatics ProcessingUser-friendly Graphic User InterfaceMolecular BiologistsCell BiologistsBiochemistsMAPster SoftwareDEEPN AnalysisHISAT2Multi-threadingReference DNA GenomeY2H Prey Library InsertsMapped FilesUnmapped Reads
