Journal
/
Biochemistry
/
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

DOI:
10.3791/57807-v

•

09:35 min

•

October 15, 2018

•

, ,
1Department of Health Science, School of Sports & Health Science,Daito Bunka University, 2Department of Molecular Life Science, School of Medicine,Tokai University, 3Department of Health Management, School of Health Study,Tokai University

Chapters

  • 00:04Title
  • 00:37Caenorhabditis elegans Synchronized Culture
  • 03:21Extraction of Cellular Fraction from C. elegans
  • 05:35Lactate, Pyruvate and Protein Assays
  • 07:32Results: Efficiency of Lactate and Pyruvate Colorimetric Assays After Protein Precipitation of C. elegans
  • 08:26Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a modified small-scale extraction and colorimetric assays of lactate and pyruvate in the nematode C. elegans. When utilizing commercial assay kits, the technical development of their sensitivity and accuracy is important. Protein precipitation in extraction is the most critical step for the quantitative determination of intracellular metabolites.

Tags

Keywords: Colorimetric AssayLactatePyruvateCaenorhabditis ElegansNematodeProtein PrecipitationHomogenizationE. Coli OP50NGM Agar PlatesGravid HermaphroditesEgg IsolationL1 LarvaeSynchronous CultureColorimetric AnalysisBiochemical Methodology

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57807?article_type=v

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
check_url/57807?article_type=v

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
check_url/57807?article_type=v

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
check_url/57807?article_type=v

A Colorimetric Method for Measuring Iron Content in Plants
check_url/57807?article_type=v

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
check_url/57807?article_type=v

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
check_url/57807?article_type=v

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
check_url/57807?article_type=v

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
check_url/57807?article_type=v

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
check_url/57807?article_type=v

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Read Article