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Journal
/
Biochemistry
/
线虫
线虫中乳酸和丙酮酸细胞的小尺度比色测定
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Biochemistry
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JoVE Journal
Biochemistry
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
线虫
线虫中乳酸和丙酮酸细胞的小尺度比色测定
DOI:
10.3791/57807-v
•
09:35 min
•
October 15, 2018
•
Sumino Yanase
2
,
Kayo Yasuda
,
Naoaki Ishii
1
Department of Health Science, School of Sports & Health Science
,
Daito Bunka University
,
2
Department of Molecular Life Science, School of Medicine
,
Tokai University
,
3
Department of Health Management, School of Health Study
,
Tokai University
Chapters
00:04
Title
00:37
Caenorhabditis elegans
Synchronized Culture
03:21
Extraction of Cellular Fraction from
C. elegans
05:35
Lactate, Pyruvate and Protein Assays
07:32
Results: Efficiency of Lactate and Pyruvate Colorimetric Assays After Protein Precipitation of
C. elegans
08:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种改良的小尺度萃取和比色法测定乳酸和丙酮酸在线虫中的含量
.
使用商用试剂盒时, 其灵敏度和准确度的技术发展非常重要。在萃取中蛋白质沉淀是定量测定细胞内代谢产物的关键步骤。
Tags
Keywords: Colorimetric Assay
Lactate
Pyruvate
Caenorhabditis Elegans
Nematode
Protein Precipitation
Homogenization
E. Coli OP50
NGM Agar Plates
Gravid Hermaphrodites
Egg Isolation
L1 Larvae
Synchronous Culture
Colorimetric Analysis
Biochemical Methodology
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