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基于气相色谱-质谱 (gc-ms) 的新陈代谢的果蝇幼虫样品的制备

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Developmental Biology

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JoVE Journal Developmental Biology

Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

Please note that all translations are automatically generated. Click here for the English version.

基于气相色谱-质谱 (gc-ms) 的新陈代谢的果蝇幼虫样品的制备

DOI:

10.3791/57847-v

•

07:21 min

•

June 06, 2018

•

Hongde Li, Jason M. Tennessen

¹Department of Biology,Indiana University

Chapters

00:05Title
00:37Larval Sample Collection
01:28Sample Transfer to Bead Tubes
02:23Sample Extraction
03:52Chemical Derivatization
06:20Results: Gas Chromatography-Mass Spectrometry Analyses Reveal Significant Differences Between Lactate Dehydrogenase Mutants and Wild-type Controls
07:02Conclusion

Summary

Automatic Translation

此协议描述如何准备果蝇幼虫进行基于 GC-MS 的 metabolomic 分析。

Tags

Keywords: Drosophila Larval Samples Gas Chromatography-mass Spectrometry (GC-MS)Metabolomics L2-hydroxyglutarate Sodium Chloride Centrifugation Liquid Nitrogen Bead Tube Methanol Succinic-D4 Acid Bead Mill Homogenizer

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