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Preparazione di campioni larvale della drosofila per spettrometria della cromatografia-massa del Gas (GC-MS)-base di metabolomica
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
DOI:

07:21 min

June 06, 2018

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Chapters

  • 00:05Title
  • 00:37Larval Sample Collection
  • 01:28Sample Transfer to Bead Tubes
  • 02:23Sample Extraction
  • 03:52Chemical Derivatization
  • 06:20Results: Gas Chromatography-Mass Spectrometry Analyses Reveal Significant Differences Between Lactate Dehydrogenase Mutants and Wild-type Controls
  • 07:02Conclusion

Summary

Automatic Translation

Questo protocollo descrive come preparare larve di Drosophila per analisi metabolomica basati su GC-MS.

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