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Journal
/
Biochemistry
/
用于膜蛋白结构分析和
从头
建模的小角中子散射实验中的对比匹配洗涤剂
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and
Ab Initio
Modeling
Please note that all translations are automatically generated.
Click here for the English version.
用于膜蛋白结构分析和
从头
建模的小角中子散射实验中的对比匹配洗涤剂
DOI:
10.3791/57901-v
•
10:27 min
•
October 21, 2018
•
Ryan C. Oliver
,
Swe-Htet Naing
,
Kevin L. Weiss
,
Sai Venkatesh Pingali
,
Raquel L. Lieberman
,
Volker S. Urban
1
Neutron Scattering Division
,
Oak Ridge National Laboratory
,
2
School of Chemistry and Biochemistry
,
Georgia Institute of Technology
Chapters
00:04
Title
00:54
Determine Neutron Contrast Match Points and Necessary Contrast for Protein Measurement
02:05
Express and Purify the Membrane Protein of Interest
03:25
Collect SANS Data and Reduce SANS Data from 2D Image to 1D Plot
04:48
Analyze Data for Structural Parameters of the Scattering Particle
07:06
Create
Ab Initio
Models from the SANS Data
08:17
Results: Visualization of the Experimental SANS Envelope and Overlay with High-resolution Model using PyMOL
09:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议演示了如何获得一个低分辨率的
从头模型和洗涤剂-溶解膜蛋白在溶液中使用小角中子散射与洗涤剂的对比匹配的结构细节。
Tags
Keywords: Membrane Protein
Small-angle Neutron Scattering
Contrast Matching
Deuterium Labeling
Ab Initio Modeling
Oligomeric State
Size
Shape
Low Resolution Structure
Detergent
Scattering Length Density
Contrast Match Point
E. Coli
Deuterated Protein
Minimal Medium
Bioreactor
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