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抗菌肽定位的细菌 Spheroplasts 和原生质体的生产与可视化
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Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
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抗菌肽定位的细菌 Spheroplasts 和原生质体的生产与可视化

DOI:
10.3791/57904-v

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10:13 min

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August 11, 2018

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, , , ,
1Biochemistry Program,Wellesley College, 2Department of Chemistry,Wellesley College, 3Department of Biological Sciences,Wellesley College

Chapters

  • 00:04Title
  • 01:14Gram-negative E. coli Spheroplast Preparation
  • 04:12Gram-positive B. megatherium Protoplast Preparation
  • 05:58<E. coli Spheroplast and B. megatherium Protoplast Visualization and Anticrobial Peptide (AMP) Localization
  • 07:49Results: Representative High-resolution AMP Visualization
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个生产革兰氏阴性大肠杆菌 (大肠杆菌) spheroplasts 和革兰氏阳性芽孢杆菌巨大(巨大) 原生质体的协议, 以清楚地形象化和快速表征多肽-细菌的相互作用。这为定义膜定位和转运肽提供了系统的方法。

Tags

Bacterial SpheroplastsBacterial ProtoplastsAntimicrobial PeptidesCell Membrane LocalizationGram-negativeGram-positiveE. ColiB. MegateriumCephalexinBacterial FilamentsCell ImagingMembrane-active AgentsCell-penetrating Peptides

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