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体内结肠细胞 Photolabeling 对新生小鼠造血细胞迁移潜能的评价
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JoVE Journal Medicine
In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice
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体内结肠细胞 Photolabeling 对新生小鼠造血细胞迁移潜能的评价

DOI:
10.3791/57929-v

•

08:39 min

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August 10, 2018

•

, ,
1Center for Computational and Integrative Biology,Massachusetts General Hospital, 2Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Chapters

  • 00:04Title
  • 00:39Colon Cell Photoconversion
  • 03:00Intestinal Lymphocytes Isolation
  • 06:49Results: Migratory Potential of Photolabeled Intestinal Cells
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

这里描述的协议使用了 photolabeling 方法在新生老鼠特别辨认免疫细胞移居从结肠到肠外的站点。这一策略将有助于研究早期生命中宿主-微生物的相互作用。

Tags

In Vivo PhotolabelingMucosal ImmunologyNeonatal MiceHematopoietic Cell MigrationColon Resident CellsOptogeneticsFiberoptic CannulaUV Light ExposureIntestinal Lymphocytes

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