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Imaging FITC-dextran as a Reporter for Regulated Exocytosis
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Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

DOI:
10.3791/57936-v

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04:50 min

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June 20, 2018

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, , , , ,
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University

Chapters

  • 00:04Title
  • 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
  • 01:52Live Cell Microscopy of Exocytosis
  • 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
  • 04:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.

Tags

Keywords: FITC-dextranRegulated ExocytosisMast Cell ExocytosisNeutrophilsEosinophilsTyrode’s BufferRBL CellsAmmonium ChlorideSecretagogueFluorescence Microscopy

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