A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University
Chapters
- 00:04Title
- 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
- 01:52Live Cell Microscopy of Exocytosis
- 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
- 04:17Conclusion
Her detalj vi en metode for levende celle avbilding av regulert exocytosis. Denne metoden bruker FITC-dekstran, som akkumuleres i lysosome-relaterte organeller, som reporter. Denne enkle metoden kan også skille mellom ulike regulert exocytosis i celler som er vanskelige å manipulere genetisk.
