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小鼠胰腺内分泌细胞的单细胞 Transcriptomic 分析

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Developmental Biology

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JoVE Journal Developmental Biology

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

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小鼠胰腺内分泌细胞的单细胞 Transcriptomic 分析

DOI:

10.3791/58000-v

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10:05 min

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September 30, 2018

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Lin-Chen Li*^1,2, Xin-Xin Yu*^1,2, Yu-Wei Zhang*¹, Ye Feng*^1,3, Wei-Lin Qiu*^1,3, Cheng-Ran Xu

¹College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, ²Academy for Advanced Interdisciplinary Studies,Peking University, ³PKU-Tsinghua-NIBS Graduate Program,Peking University

Chapters

00:04Title
01:05Pancreas Isolation
03:04Tissue Digestion and Islet Isolation
04:53Single-cell Lysis and cDNA Amplification
07:32Results: Single-cell RNA-Seq of Mouse Pancreatic Endocrine Cells
09:13Conclusion

Summary

Automatic Translation

我们描述了一种方法, 从胚胎, 新生儿和产后胰腺的内分泌细胞分离, 其次是单细胞 RNA 测序。这种方法可以分析胰腺内分泌谱系的发展, 细胞异质性和 transcriptomic 动力学。

Tags

Single-cell Transcriptomics Pancreatic Endocrine Cells Lineage Differentiation Maturation Regeneration Diabetes Pancreatic Endocrine Diseases Collagenase Perfusion Bile Duct Mouse Pancreas

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