Journal
/
/
Encellede Transcriptomic analyser av musen bukspyttkjertelen endokrine celler
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
DOI:

10:05 min

September 30, 2018

, , , , ,

Chapters

  • 00:04Title
  • 01:05Pancreas Isolation
  • 03:04Tissue Digestion and Islet Isolation
  • 04:53Single-cell Lysis and cDNA Amplification
  • 07:32Results: Single-cell RNA-Seq of Mouse Pancreatic Endocrine Cells
  • 09:13Conclusion

Summary

Automatic Translation

Vi beskriver en metode for isolering av endokrine celler fra embryonale, neonatal og postnatal pancreases etterfulgt av encellede RNA sekvensering. Denne metoden kan analyser av bukspyttkjertelen endokrine avstamning utvikling, celle heterogenitet og transcriptomic dynamics.

Related Videos

Read Article