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Necroptosis MLKL 介导的血浆膜破裂特征

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Biology

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JoVE Journal Biology

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

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Necroptosis MLKL 介导的血浆膜破裂特征

DOI:

10.3791/58088-v

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08:55 min

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August 07, 2018

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Dan E. McNamara*^1,2, Giovanni Quarato*³, Cliff S. Guy, Douglas R. Green, Tudor Moldoveanu²

¹Department of Structural Biology,St. Jude Children’s Research Hospital, ²Department of Chemical Biology and Therapeutics,St. Jude Children’s Research Hospital, ³Department of Immunology,St. Jude Children’s Research Hospital

Chapters

00:04Title
00:50Mixed Lineage Kinase Domain-like (MLKL)-mediated Necroptosis Live-cell Microscopy Imaging
03:26Nuclear Magnetic Resonance (NMR) Spectroscopy MLKL Lipid Binding
05:35Results: Representative MLKL: Plasma Membrane Interaction Analyses
07:26Conclusion

Summary

Automatic Translation

我们报告的方法, MLKL 介导的等离子膜破裂在 necroptosis 包括常规和共聚焦活细胞显微成像, 扫描电镜, 核磁共振基脂结合。

Tags

Keywords: MLKL Necroptosis Plasma Membrane Rupture Live-cell Imaging Nuclear Magnetic Resonance Lipid Binding Membrane Translocation Permeabilization Mouse Embryonic Fibroblasts Doxycycline FK506-binding Protein Dimerizer Fluorescence Microscopy Cell Death Quantification

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