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Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
 

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Article DOI: 10.3791/58099-v • 09:14 min • August 12th, 2018
August 12th, 2018 •
*1,2, *1,2, 3, 1,2
1Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, 2Department of Biochemistry, College of Medicine, Dong-A University, 3Aging Institute, Department of Medicine, University of Pittsburgh School of Medicine
* These authors contributed equally

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Summary
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Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.

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Keywords: Mitophagy Flow Cytometry Mt-Keima CCCP HeLa Cells Mitochondria Lentivirus Puromycin
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