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Immunology and Infection

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小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1 分化的体外模型研究

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Immunology and Infection

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JoVE Journal Immunology and Infection

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

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小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1 分化的体外模型研究

DOI:

10.3791/58118-v

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08:39 min

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August 22, 2018

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Raquel Toribio-Fernandez, Virginia Zorita, Beatriz Herrero-Fernandez, Jose M. Gonzalez-Granado^2,3

¹LamImSys Lab,Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ²LamImSys Lab,Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), ³CIBER de Enfermedades Cardiovasculares

Chapters

00:04Title
00:46Mouse Bone Marrow (BM) and Secondary Lymphoid Tissue Cell Isolation
03:40In Vivo Activation, Proliferation, and Th1 Cell Differentiation Assay
06:48Results: Representative BM-derived Dentritic Cell-induced Naïve CD4+ T Cell Activation, Proliferation, and Th1 Differentiation
07:39Conclusion

Summary

Automatic Translation

在这里, 我们提出了一种在体内测定由 GM-脑脊液骨髓 (BM) 衍生树突状细胞 (CD4) 诱导的天真的 t 细胞 (t 细胞) 活化、增殖和 Th1 分化的方法。此外, 该协议还描述了 BM 和 t 细胞分离、dc 生成和 dc 和 t 细胞的移植。

Tags

In Vivo Mouse Model Naive CD4 T Cell Activation Proliferation Th1 Differentiation Bone Marrow-derived Dendritic Cells T-cell Activation Adaptive Immunity Antigen Presenting Cell Bone Marrow Cell Isolation Lymph Node Isolation Spleen Isolation OT-II Transgenic Mice

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