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Saccharomyces cerevisiae Eksponentiel vækst kinetik i Batch kultur til at analysere respiratorisk og Fermentativ stofskifte
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JoVE Journal Biology
Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Saccharomyces cerevisiae Eksponentiel vækst kinetik i Batch kultur til at analysere respiratorisk og Fermentativ stofskifte

DOI:
10.3791/58192-v

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07:38 min

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September 30, 2018

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, , ,
1Department of Chemistry,Universidad Autónoma de Querétaro, 2Department of Biochemical Engineering,Instituto Tecnológico de Morelia, 3Department of Biochemical Engineering,Instituto Tecnológico Superior de Ciudad Hidalgo

Chapters

  • 00:04Title
  • 00:31Inoculum Preparation and Growth Curves
  • 03:09Data Processing and Kinetic Parameter Calculation
  • 05:41Results: Representative Exponential Growth Kinetic Analysis
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol for at anslå den respiratoriske og Fermentativ metabolisme ved at montere den eksponentielle vækst af Saccharomyces cerevisiae eksponentiel vækst ligningen. Beregning af de kinetiske parametre giver mulighed for screening af påvirkninger af stoffer/forbindelser på gæring eller mitokondrie respiration.

Tags

Saccharomyces CerevisiaeExponential Growth KineticsBatch CultureRespiratory MetabolismFermentative MetabolismWarburg EffectCrabtree EffectYeast Extract Peptone Dextrose (YPD) BrothGlycerol PreservationAgar PlateMicroplate Growth CurveShaken Conical FlaskOptical DensityAgitation Speed

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