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Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles
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Cancer Research
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JoVE Journal Cancer Research
Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles

Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles

DOI:
10.3791/58209-v

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05:55 min

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September 18, 2018

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, , ,
1Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS), 2Guangdong Key Laboratory of Nanomedicine, CAS Key Lab for Health Informatics,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS), 3Department of Chemistry,Guangdong Medical University

Chapters

  • 00:04Title
  • 00:35Synthesis of DNPs
  • 01:34Conjugation of Peptides to DNPs
  • 02:54Fluorescence Microscopy Assessment of pICSA-Targeted Nanoparticle Uptake in Choriocarcinoma (JEG3) Cells
  • 04:18Results: Characterization and Cellular Uptake of plCSA-DNPs
  • 05:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the synthesis of placental chondroitin sulfate A binding peptide (plCSA-BP)-conjugated lipid-polymer nanoparticles via single-step sonication and bioconjugate techniques. These particles constitute a novel tool for the targeted delivery of therapeutics to most human tumors and placental trophoblasts to treat cancers and placental disorders.

Tags

Keywords: Placental Chondroitin Sulfate APlCSALipid-polymer NanoparticlesTargeted Drug DeliveryPLGADOXEDCNHSAmine ReactionDialysisCell CultureNanoparticle Uptake

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