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Reprimiendo la transcripción genética redirigiendo la maquinaria celular con modificadores químicos de epigenéticas
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1Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, Curriculum in Genetics and Molecular Biology,University of North Carolina, 2College of Arts and Sciences, Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery,University of North Carolina, 3Chemical Biology and Drug Discovery, Pharmacological Sciences and Oncological Sciences,Icahn School of Medicine at Mount Sinai, 4Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center,University of North Carolina
Chapters
- 00:04Title
- 00:49Cell Line Culture for Lentivirus Production
- 02:27Lentiviral Infection of Mouse Embryonic Stem Cells (mESCs)
- 05:28Chemical Epigenetic Modifiers (CEM)s Preparation and Treatment
- 06:47Microscopic and Flow Cytometry Analysis of Expression
- 08:55Results: Effective Gene Repression Using Chemical Epigenetic Modifiers
- 10:00Conclusion
Reglamento de medio ambiente de la cromatina es un proceso esencial para expresión génica adecuada. Aquí, describimos un método para controlar la expresión génica a través de la contratación de modificadores de la cromatina maquinaria de manera gene-específica y reversible.
