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利用 Cas9 Ribonucleoproteins 生成人 NK 细胞的研究

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Immunology and Infection

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JoVE Journal Immunology and Infection

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Please note that all translations are automatically generated. Click here for the English version.

利用 Cas9 Ribonucleoproteins 生成人 NK 细胞的研究

DOI:

10.3791/58237-v

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07:20 min

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June 14, 2018

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Meisam Naeimi Kararoudi, Hamid Dolatshad, Prashant Trikha, Syed-Rehan A. Hussain, Ezgi Elmas, Jennifer A. Foltz, Jena E. Moseman, Aarohi Thakkar, Robin J. Nakkula, Margaret Lamb, Nitin Chakravarti, K. John McLaughlin, Dean A. Lee

¹Center for Childhood Cancer and Blood Disease,Nationwide Children’s Hospital, ²Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, ³Center for Clinical and Translational Research,Nationwide Children’s Hospital Research Institute

Chapters

00:04Title
00:44Human Primary and Expanded Natural Killer (NK) Cell Transduction
04:48Results: Representative Electroporation Efficiency
06:13Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 基因改造主要或扩大人类自然杀手 (NK) 细胞使用Cas9 Ribonucleoproteins (Cas9/RNPs)。通过使用该协议, 我们生成的人 NK 细胞缺乏转化生长 factor–b 受体 2 (TGFBR2) 和嘌呤 phosphoribosyltransferase 1 (HPRT1)。

Tags

Cas9 Ribonucleoproteins NK Cells Gene Knockout Primary NK Cells Expanded NK Cells CRISPR RNA TracrRNA Electroporation Transduction Immunotherapy Solid Tumors Brain Tumors TGF-beta

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