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用水凝胶微室阵列 (HMCA) 板进行肿瘤细胞侵袭和抗转移药物筛选的分析

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Cancer Research

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JoVE Journal Cancer Research

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates

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用水凝胶微室阵列 (HMCA) 板进行肿瘤细胞侵袭和抗转移药物筛选的分析

DOI:

10.3791/58359-v

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08:32 min

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October 25, 2018

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Orit Ravid-Hermesh, Naomi Zurgil, Yana Shafran, Elena Afrimzon, Maria Sobolev, Yaron Hakuk, Zehavit Bar-On Eizig, Mordechai Deutsch

¹Physics Department,Bar-Ilan University

Chapters

00:04Title
01:17Hyrdogel Micro-chamber Array (HMCA) Plate Embossing
02:28Cell Loading and Spheroid Formation
04:26Invasion Assay Acquisition and Analysis
06:06Results: Representative Influence of Extracellular Matrix (ECM) Composition on Spheroid Invasion
07:25Conclusion

Summary

Automatic Translation

针对入侵检测的性能, 提出了一种基于 HMCA 的成像板。该板块有助于形成三维 (3D) 肿瘤旋转椭球体和测量癌细胞侵入细胞外基质 (ECM)。通过半自动分析实现了入侵检测的定量化。

Tags

Keywords: 3D Cell Culture Hydrogel Micro-chamber Array Tumor Spheroid Anti-metastatic Drug Screening Cell Invasion PDMS Stamp Agarose Gel UV Sterilization Cell Seeding

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