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En In Vivo metode til evaluering af Gut-blod barriere og lever metabolisme af mikrobiota produkter
JoVE Journal
Medicine
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JoVE Journal Medicine
An In Vivo Method for Evaluating the Gut-Blood Barrier and Liver Metabolism of Microbiota Products
DOI:

14:54 min

October 20, 2018

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Chapters

  • 00:00Title
  • 02:17Procedure 1: Insertion of the Line for Intraintestinal Administration
  • 03:57Procedure 2: Inferior Vena Cava Catheterization
  • 06:44Procedure 3: Portal Vein Catheterization
  • 10:46Procedure 4: Portal Vein Blood Sampling
  • 11:22Procedure 5: Inferior Vena Cava Blood Sampling
  • 11:51Procedure 6: Administration of Permeability Marker
  • 12:59Procedure 7: Calculation of Hepatic Clearance
  • 13:20Results
  • 14:16Conclusion

Summary

Automatic Translation

Næringsstoffer, mikrobiota metabolitter og lægemidler til cirkulation adgang styres af gut-blod barriere (GBB). Vi beskriver en direkte metode til måling af GBB permeabilitet i vivo, der i modsætning til almindeligt anvendte indirekte metoder, er stort set ikke berørt af lever og nyre funktioner.

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