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Gen Expression analyse i Endothelial celler udsat for Shear Stress ved hjælp af flere Parallel-plade Flow kamre
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
DOI:

08:50 min

October 21, 2018

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Chapters

  • 00:04Title
  • 00:55Assay Setup
  • 04:20Flow Assay
  • 05:18Cell Harvest and RNA Extraction
  • 06:48Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress
  • 08:06Conclusion

Summary

Automatic Translation

Her præsenteres en arbejdsproces for kultur og gen expression analysen i endothelial celler under væske shear stress. Inkluderet er en fysisk arrangement for samtidig boliger og overvågning flere flow kamre i et kontrolleret miljø og brugen af en udefrakommende reference RNA til kvantitativ PCR.

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