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Immunology and Infection

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肿瘤切片提取物的建立与分析是诊断检测的前提

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

Please note that all translations are automatically generated. Click here for the English version.

肿瘤切片提取物的建立与分析是诊断检测的前提

DOI:

10.3791/58569-v

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11:00 min

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November 29, 2018

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Ashwini S. Nagaraj, Jie Bao, Annabrita Hemmes, Mafalda Machado, Katja Närhi, Emmy W. Verschuren

¹Institute for Molecular Medicine Finland (FIMM), HiLIFE,University of Helsinki

Chapters

00:04Title
00:42Collection of Tumor-bearing Lungs
02:08Generation and Cultivation of Precision-cut Lung Tumor Slices, and Treatment with Inhibitors
05:58Fixation and Processing of the Tissue Slices
07:27Processing and Analysis of Formalin-fixed and Paraffin-embedded (FFPE) Tissues
08:33Results: Assessment of Viability, Specific Marker Expression, and Targeted Drug Treatment on Adenocarcinoma Tissue Slices
10:26Conclusion

Summary

Automatic Translation

为小鼠肺肿瘤的组织型切片的生成、培养和系统分析提供了一种方法。我们还描述了如何优化切片厚度, 以及如何选择药物浓度来治疗肿瘤切片。

Tags

Tumor Slice Explants Preclinical Research Drug Response Studies Murine Lung Tumors Vibratome Hank’s Balanced Salt Solution Euthanized Mouse Lung Heart Cyanoacrylate Adhesive

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