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Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

DOI:
10.3791/58603-v

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10:09 min

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November 06, 2018

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, ,
1Department of Ocean Science,Hong Kong University of Science and Technology, 2Division of Life Science,Hong Kong University of Science and Technology, 3HKUST Shenzhen Research Institute

Chapters

  • 00:04Title
  • 00:43Sample Preparation and Fixation
  • 01:44Precoating of the Coverslip with Polystyrene Beads and Coating of Poly-L-lysine onto the Bead-coated Coverslip
  • 03:08Immobilization of Cells on the Coverslip and Permeabilization of Cyanobacterial Cells
  • 04:23Photobleaching of the Chlorophyll Pigments in a Blocking Step
  • 04:51Antibody Binding
  • 06:07Image Acquisition of STORM Data
  • 07:51Results: Super-resolution Imaging of the FtsZ Ringin in the Cyanobacterium Prochlorococcus
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a photobleaching method to reduce the autofluorescence of cyanobacteria. After photobleaching, stochastic optical reconstruction microscopy is used to obtain three-dimensional super-resolution images of the cyanobacterial FtsZ ring.

Tags

Keywords: PhotobleachingSuper-resolution ImagingFtsZ RingProchlorococcusProtein LocalizationProtein DynamicsPhotosynthetic OrganismsRadio-re-dop-singBacteriochlorophyllProchlorococcus MED4FormaldehydeImmunostainingPolystyrene BeadsPoly-L-lysine

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