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Bioengineering
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3D 动态培养系统构建多层间充质干细胞片材
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
Please note that all translations are automatically generated.
Click here for the English version.
3D 动态培养系统构建多层间充质干细胞片材
DOI:
10.3791/58624-v
•
07:44 min
•
October 20, 2018
•
Yingwei Wang*
1,2
,
Cheng Lu*
1,2
,
Chengzhi He
,
Baoxin Chen
2
,
Youling Zheng
2
,
Junming Zheng
2
,
Jianhua Zhang
,
Zheng Wu
2
1
Key Laboratory for Regenerative Medicine, Ministry of Education
,
Jinan University
,
2
Department of Developmental and Regenerative Biology
,
Jinan University
,
3
Nansha College Preparatory Academy
,
4
Department of Cardiology
,
First Affiliated Hospital of Jinan University
Chapters
00:00
Title
01:02
Preparations Before Cell Sheet Construction
01:39
Preparing Cells for Cell Sheet Construction
03:22
Preparing BMSCs and RAD16-I Peptide Hydrogel Mixture
04:36
In vitro
Culture of 3D Multilayered Cell Sheet Using a Dynamic Culture System
05:18
Obtain the Multilayered MSC Cell Sheet
05:49
Results
06:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文为构建具有良好干细胞性质的多层干细胞片提供了一种高效可行的方法。
Tags
Keywords: Mesenchymal Stem Cell
Cell Sheet
3D Dynamic Culture System
Multilayered Stem Cell Sheet
Pericardium Scaffold
Nonapeptide Hydrogel
Cell Nutrition Supply
Cell Retention
Cell Survival
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