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板基检测血液中一氧化氮的 vasp 磷酸化
JoVE Journal
Medicine
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JoVE Journal Medicine
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
DOI:

07:13 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:45Blood Sample Preparation and Deoxygenation
  • 02:52Red Blood Cell (RBC) Nitrite Reduction and Vasodilator-stimulated Phosphoprotein (VASP) Western Blotting
  • 04:39Results: Representative Platelet-based Nitric Oxide (NO) Detection
  • 06:18Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以解决潜在的使用血小板作为一个高度敏感的一氧化氮传感器在血液中。它描述了最初的血小板制备和使用亚硝酸盐和脱氧红细胞作为一氧化氮发生器。

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